Dialysis is a process of separating colloids from crystalloids by using a semipermeable membrane. The membrane allows the passage of solvent and crystalloids, but it retains the colloids. The colloids are retained because their particles are too large to pass through the pores of the membrane.
Procedure:
- The colloid is placed in a dialysis bag made of semipermeable membrane.
- The dialysis bag is suspended in a beaker of water.
- The water in the beaker is stirred gently.
- The solvent and crystalloids diffuse through the membrane into the dialysis bag.
- The colloids remain in the dialysis bag.
2. Ultrafiltration
Ultrafiltration is a process of separating colloids from crystalloids by using a semipermeable membrane under pressure. The pressure forces the solvent and crystalloids through the membrane, but it retains the colloids. The colloids are retained because their particles are too large to pass through the pores of the membrane.
Procedure:
- The colloid is placed in a chamber that is fitted with a semipermeable membrane.
- Pressure is applied to the colloid.
- The solvent and crystalloids are forced through the membrane into a filtrate.
- The colloids remain in the chamber.
3. Centrifugation
Centrifugation is a process of separating colloids from crystalloids by using a centrifuge. The centrifuge applies centrifugal force to the colloid, which causes the colloids to sediment at the bottom of the tube. The crystalloids remain in the supernatant.
Procedure:
- The colloid is placed in a centrifuge tube.
- The centrifuge tube is placed in a centrifuge.
- The centrifuge is turned on and the speed is gradually increased.
- The colloids sediment at the bottom of the tube.
- The supernatant is decanted.
4. Electrophoresis
Electrophoresis is a process of separating colloids from crystalloids by using an electric field. The electric field causes the colloids to migrate towards the oppositely charged electrode. The colloids migrate at different rates depending on their size and charge.
Procedure:
- The colloid is placed in a chamber that is fitted with two electrodes.
- An electric field is applied to the chamber.
- The colloids migrate towards the oppositely charged electrode.
- The colloids are collected at the electrode.