1. Glycolysis: This first step of cellular respiration occurs in the cytoplasm, not the mitochondria. Here, glucose is broken down into pyruvate.
2. Krebs Cycle (Citric Acid Cycle):
- Pyruvate from glycolysis enters the mitochondria and is further broken down.
- The Krebs cycle generates high-energy electron carriers (NADH and FADH2) and some ATP.
3. Electron Transport Chain (ETC):
- The electron carriers (NADH and FADH2) from the Krebs cycle deliver electrons to a series of protein complexes embedded in the inner mitochondrial membrane.
- As electrons pass through these complexes, energy is released and used to pump protons (H+) across the membrane, creating a proton gradient.
- The flow of protons back across the membrane drives the production of ATP, the cell's primary energy currency.
In summary, mitochondria are the site of:
* ATP production: The main function of mitochondria is to generate ATP through oxidative phosphorylation, which is powered by the ETC and proton gradient.
* Krebs Cycle: This cycle breaks down pyruvate, producing electron carriers and some ATP.
* Other metabolic functions: Mitochondria also play roles in:
* Amino acid metabolism
* Fatty acid metabolism
* Calcium signaling
* Apoptosis (programmed cell death)
Important Note: Mitochondria have their own DNA (mtDNA) and are thought to have originated from bacteria that were engulfed by eukaryotic cells billions of years ago. This explains why mitochondria have their own unique set of genes and can replicate independently within the cell.