The second generation of offspring from a cross of two organisms is called the F2 generation.

Here's a breakdown of the terminology:

* P generation: The parental generation, the original two organisms that are crossed.

* F1 generation: The first filial generation, the offspring resulting from the cross of the P generation.

* F2 generation: The second filial generation, the offspring resulting from a cross between two individuals from the F1 generation.

This terminology is commonly used in genetics, particularly when studying Mendelian inheritance patterns.