1. Cell type: Are the cells prokaryotic (lacking a nucleus and other membrane-bound organelles) or eukaryotic (having a nucleus and other membrane-bound organelles)? This is a fundamental difference that separates the kingdoms Bacteria and Archaea from the other kingdoms.
2. Cell structure: Beyond just being prokaryotic or eukaryotic, how are the cells organized? Do they have cell walls? How many cells do they have (single-celled or multicellular)? What types of organelles are present?
3. Nutrition: How does the organism obtain its energy? Is it a producer (photosynthetic), a consumer (eating other organisms), or a decomposer (breaking down dead organic matter)?
4. Mode of reproduction: How does the organism reproduce? Is it sexual or asexual? Does it have a complex life cycle?
It's important to note that the classification system is constantly evolving, and there are still debates about how best to categorize organisms. These four characteristics are broadly used, but other factors like evolutionary history and specific features within a kingdom can also play a role in classification.