Before utilizing eDNA for invasive species detection, the researchers should first select the target species they are looking for and collect reference samples. This involves obtaining known DNA samples from the invasive species of interest. The reference samples are crucial for creating an eDNA reference database, which will be used to compare against the eDNA collected from the waterway. Having accurate and representative reference samples is a critical step in ensuring the effectiveness and specificity of eDNA surveillance for the target invasive species.