Calculation:
First, determine the dilution factor required to achieve the desired concentration. The dilution factor can be calculated using the formula:
Dilution Factor = (Concentration of Stock Solution) / (Desired Concentration)
In this case, the concentration of the stock solution is 1 Molar, and the desired concentration is 3.5 millimolar (mM). So, the dilution factor is:
Dilution Factor = (1 M) / (3.5 mM) = 285.7
This means that you need to dilute the stock solution by a factor of 285.7 to obtain a 3.5 mM solution.
Step-by-Step Instructions:
1. Prepare Materials:
- 1 Molar Sulfuric Acid Stock Solution
- Deionized or Distilled Water
- Pipette or Micropipette
- Graduated Cylinder or Volumetric Flask
2. Calculate the Volume of Stock Solution Needed:
- To calculate the volume of stock solution required, divide the desired final volume of the 3.5 mM solution by the dilution factor.
- Let's assume you want to prepare 100 mL of the 3.5 mM solution.
Volume of Stock Solution = (Final Volume) / (Dilution Factor)
= (100 mL) / (285.7)
= 0.35 mL
3. Prepare the 3.5 mM Solution:
- Using a pipette or micropipette, carefully transfer 0.35 mL of the 1 M sulfuric acid stock solution into a graduated cylinder or volumetric flask.
- Add deionized or distilled water to the graduated cylinder or volumetric flask up to the 100 mL mark.
- Mix the solution thoroughly to ensure uniform concentration.
Your 3.5 millimolar sulfuric acid solution is now ready to use.
