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  • 33P and Nucleotide Synthesis: A Guide to Radioisotope Labeling
    Yes, 33P can be used to make nucleotides. Nucleotides are the building blocks of nucleic acids, such as DNA and RNA. They consist of a nitrogenous base, a ribose or deoxyribose sugar, and a phosphate group. 33P can be incorporated into nucleotides by replacing the normal phosphorus atom in the phosphate group. This can be done by using 33P-labeled phosphate salts in the synthesis of nucleotides. The resulting 33P-labeled nucleotides can then be used to study the metabolism and function of nucleic acids.
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