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  • DNA Ligase: The Enzyme That Joins Okazaki Fragments During Replication
    The enzyme used to join the DNA strand that is replicated in small segments is called DNA ligase.

    Here's why:

    * DNA replication in eukaryotes is discontinuous on the lagging strand. This means that it is synthesized in small fragments called Okazaki fragments.

    * DNA polymerase can only add nucleotides to the 3' end of a pre-existing strand. This means it can't create a new strand from scratch.

    * DNA ligase comes in to fill the gaps between the Okazaki fragments, creating a continuous strand of DNA.

    So, while DNA polymerase is responsible for the actual synthesis of the DNA strand, DNA ligase is crucial for joining the fragments together to form a complete, continuous strand.

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