1. mRNA Binding:
- mRNA, which carries the genetic code from the DNA, binds to the small subunit of the ribosome.
2. tRNA Binding:
- Transfer RNA (tRNA) molecules, each carrying a specific amino acid, bind to the mRNA at a specific codon (a three-nucleotide sequence) according to the genetic code.
3. Peptide Bond Formation:
- The ribosome catalyzes the formation of a peptide bond between the amino acids carried by the tRNAs, linking them together in a chain.
4. Translocation:
- The ribosome moves along the mRNA, reading the next codon and bringing in the next tRNA with its corresponding amino acid. The process continues, elongating the polypeptide chain.
5. Termination:
- When the ribosome encounters a stop codon on the mRNA, the polypeptide chain is released, and the ribosome disassembles.
In summary, the key events at the ribosome are:
* Decoding mRNA: Reading the genetic code on mRNA.
* Recruiting tRNAs: Bringing in the correct tRNAs based on the codons.
* Peptide bond formation: Connecting amino acids to form a polypeptide chain.
* Translocation: Moving along the mRNA to read the next codon.
* Termination: Releasing the completed polypeptide chain.
This entire process is called translation, which is a fundamental process of life, essential for building and maintaining all living organisms.