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  • Understanding Sticky Ends: DNA Fragments After Restriction Enzyme Cleavage
    The unpaired nucleotides produced by the action of restriction enzymes are referred to as sticky ends.

    Here's why:

    * Restriction enzymes cut DNA at specific sequences, often leaving short, single-stranded overhangs.

    * These overhangs are "sticky" because they can base pair with complementary overhangs on other DNA fragments that have been cut by the same enzyme.

    * This ability to form complementary base pairs makes sticky ends crucial for ligation, the process of joining DNA fragments together.

    Let me know if you have any other questions!

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