1. Transcription:
* Location: Occurs in the nucleus of eukaryotic cells.
* Process: The genetic code stored in DNA is copied into a messenger RNA (mRNA) molecule.
* Mechanism: The DNA double helix unwinds, and an enzyme called RNA polymerase reads the DNA sequence. RNA polymerase uses this sequence to assemble a complementary mRNA molecule, using uracil (U) instead of thymine (T).
* Result: A messenger RNA (mRNA) molecule is created, carrying the genetic code from DNA to the ribosomes.
2. Translation:
* Location: Occurs in the cytoplasm of the cell, specifically at ribosomes.
* Process: The mRNA molecule is used as a template to assemble amino acids into a polypeptide chain, which folds into a functional protein.
* Mechanism:
* Ribosomes bind to the mRNA molecule.
* Transfer RNA (tRNA) molecules, each carrying a specific amino acid, recognize and bind to codons (sequences of three nucleotides) on the mRNA.
* The ribosome moves along the mRNA, linking amino acids together in the order specified by the codons.
* This process continues until a stop codon is reached, signaling the end of the protein chain.
* Result: A polypeptide chain is created, which folds into a functional protein.
Overall, protein synthesis is the process of converting the genetic information stored in DNA into functional proteins, essential for various cellular processes and functions.