One key difference in the DNA of prokaryotic cells compared to eukaryotic cells is that prokaryotic DNA is circular, while eukaryotic DNA is linear.

Here's a breakdown:

* Prokaryotes: Their DNA is found in a single, circular chromosome located in a region called the nucleoid. This chromosome is not enclosed within a membrane-bound nucleus.

* Eukaryotes: Their DNA is organized into multiple linear chromosomes, each contained within a membrane-bound nucleus.

This difference in structure has implications for how DNA is replicated and organized within the cell.