Ligation
Ligation is the process of joining the recombinant DNA (containing the gene of interest) with the vector DNA. This is done using an enzyme called DNA ligase, which forms phosphodiester bonds between the ends of the DNA fragments.
Here's why ligation is crucial:
* Joining the gene and vector: Ligation ensures that the gene of interest is inserted into the vector's DNA sequence. This creates a stable molecule that can be introduced into the host cell.
* Creating a functional construct: The vector often contains sequences necessary for replication and expression of the inserted gene within the host cell. Ligation connects these sequences with the gene of interest, ensuring the gene can be copied and transcribed within the host.
In summary: Ligation is the essential process that links the recombinant DNA with the vector, creating a functional unit that can be introduced into a host cell for gene expression.
