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  • DNA Replication: Leading Strand Synthesis Direction - 5' to 3'
    The direction of DNA replication on the leading strand occurs in the 5' to 3' direction.

    Here's why:

    * DNA polymerase, the enzyme responsible for building the new DNA strand, can only add nucleotides to the 3' hydroxyl (-OH) group of the existing strand.

    * The leading strand is synthesized continuously in the same direction as the unwinding of the DNA helix, which means it follows the movement of the replication fork.

    * The 5' end of the leading strand is the end that is exposed as the helix unwinds, and the 3' end is the end that is growing.

    * Therefore, DNA polymerase can only add nucleotides to the 3' end of the leading strand, resulting in replication happening in the 5' to 3' direction.

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