Here's a breakdown:
* DNA Template Strand: During transcription, RNA polymerase uses one strand of DNA as a template to build a complementary RNA molecule. This strand is called the template strand.
* Direction of Movement: RNA polymerase moves along the template strand in a 3' to 5' direction. This is because it adds nucleotides to the growing RNA molecule at its 3' end.
* RNA Molecule Synthesis: As RNA polymerase moves, it reads the template strand and builds the RNA molecule in a 5' to 3' direction.
Think of it like this:
* DNA: The blueprint.
* RNA polymerase: The builder.
* Template strand: The blueprint's instructions.
* RNA: The building material.
The RNA polymerase "reads" the template strand and uses its information to create a new RNA molecule.