No, red blood cells of chickens, like those of mammals, do not have a nucleus. They are nucleated meaning they lack a nucleus.

This is unlike the red blood cells of most other vertebrates, such as amphibians, reptiles, and fish, whose red blood cells do contain a nucleus.

The absence of a nucleus in avian and mammalian red blood cells allows for more space to carry oxygen. The lack of a nucleus also increases the red blood cells' flexibility, allowing them to squeeze through narrow capillaries.