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  • DNA Migration in Electrophoresis: Understanding Negative Charge
    The property of DNA that causes it to migrate towards the positive pole (not opposite) in an electrophoresis apparatus is its negative charge.

    Here's why:

    * DNA's Phosphate Backbone: DNA is made up of a sugar-phosphate backbone. The phosphate groups in this backbone are negatively charged.

    * Electrophoresis: Electrophoresis uses an electric field to separate molecules based on their size and charge. The apparatus has a positive electrode at one end and a negative electrode at the other.

    * Migration: Since DNA is negatively charged, it is attracted to the positive electrode. This causes it to move towards the positive pole during electrophoresis.

    Note: Smaller DNA fragments migrate faster than larger ones because they experience less resistance from the gel matrix.

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