1. Transcription: DNA, which contains the genetic information, is transcribed into mRNA in the nucleus. During transcription, an enzyme called RNA polymerase reads the DNA sequence and synthesizes a complementary mRNA molecule.
2. Processing: The primary mRNA transcript undergoes processing to become mature mRNA. This includes removing non-coding regions (introns) and splicing together the coding regions (exons).
3. Translation: Mature mRNA then moves to the cytoplasm, where it serves as a template for protein synthesis. The process of translation involves the decoding of the mRNA sequence by ribosomes.
4. tRNA Involvement: Transfer RNA (tRNA) molecules play a crucial role in translation. Each tRNA molecule carries a specific amino acid and recognizes the corresponding codon (a sequence of three nucleotides) on the mRNA.
So, in the overall process of protein synthesis, mRNA comes first and carries the genetic information from DNA to the ribosome, where tRNA molecules bring the appropriate amino acids to be linked together into a polypeptide chain.